Table 2 Variables are showing observed values of periplasmic and cytoplasmic hGH hormone expression
Run | IPTG (mM) | Temperature ( ËšC) | Cell density at induction time (OD600) | Post induction time (H) | Cytoplasmic Production (ug/ml) | Periplasmic Production (ug/ml) |
|---|---|---|---|---|---|---|
1 | 1 | 20 | 1.5 | 16 | 42 | 60 |
2 | 0.2 | 33 | 1.5 | 16 | 20 | 60 |
3 | 0.2 | 20 | 0.5 | 4 | 0 | 0 |
4 | 0.6 | 26.5 | 1 | 4 | 0 | 0 |
5 | 0.6 | 26.5 | 0.5 | 10 | 0 | 0 |
6 | 1.3 | 26.5 | 1 | 10 | 20 | 0 |
7 | 1 | 33 | 1.5 | 4 | 19 | 90 |
8 | 1 | 33 | 0.5 | 4 | 0 | 0 |
9 | 0.2 | 26.5 | 1 | 10 | 32 | 30 |
10 | 0.6 | 26.5 | 1.5 | 10 | 33 | 60 |
11 | 0.6 | 20 | 1 | 10 | 9 | 26 |
12 | 0.6 | 26.5 | 1 | 10 | 35 | 100 |
13 | 1 | 20 | 0.5 | 16 | 19 | 0 |
14 | 0.6 | 26.5 | 1 | 16 | 46 | 84 |
15 | 0.6 | 26.5 | 1 | 10 | 44 | 95 |
16 | 0.6 | 26.5 | 1 | 10 | 45 | 110 |
17 | 0.2 | 33 | 0.5 | 16 | 60 | 147 |
18 | 0.2 | 20 | 1.5 | 4 | 58 | 54 |
19 | 0.6 | 33 | 1 | 10 | 39 | 76 |
20 | 0.6 | 26.5 | 1 | 10 | 28 | 34 |
21 | 0.6 | 26.5 | 1 | 10 | 16 | 26 |