Skip to main content

Table 2 Variables are showing observed values of periplasmic and cytoplasmic hGH hormone expression

From: Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Run IPTG
(mM)
Temperature ( ˚C) Cell density at induction time
(OD600)
Post induction time
(H)
Cytoplasmic Production (ug/ml) Periplasmic Production (ug/ml)
1 1 20 1.5 16 42 60
2 0.2 33 1.5 16 20 60
3 0.2 20 0.5 4 0 0
4 0.6 26.5 1 4 0 0
5 0.6 26.5 0.5 10 0 0
6 1.3 26.5 1 10 20 0
7 1 33 1.5 4 19 90
8 1 33 0.5 4 0 0
9 0.2 26.5 1 10 32 30
10 0.6 26.5 1.5 10 33 60
11 0.6 20 1 10 9 26
12 0.6 26.5 1 10 35 100
13 1 20 0.5 16 19 0
14 0.6 26.5 1 16 46 84
15 0.6 26.5 1 10 44 95
16 0.6 26.5 1 10 45 110
17 0.2 33 0.5 16 60 147
18 0.2 20 1.5 4 58 54
19 0.6 33 1 10 39 76
20 0.6 26.5 1 10 28 34
21 0.6 26.5 1 10 16 26