Fig. 1
From: The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitro
The expression of recombinant sHSPB5-Fc protein. A, Schematic of the sHSPB5-Fc construct. SP: signal peptide. Fc: Fc-tag. B, Immunoblot of the expression of HSPB5-Fc in the supernatant and cell lysates with antibodies against the Fc-tag and HSPB5. Lane 1: CHO-K1 cells with empty vector; lanes 2–4: CHO-K1 cells expressing three psHSPB5-Fc constructs (#1, #2, #3) respectively. C, Coomassie blue stain of sHSPB5-Fc protein purified from CHO-K1 supernatant (psHSPB5-Fc #2 construct). Supernatants: 1 and 2 represented non purified sHSBP5-Fc in supernatants. Elution 1 and 2: represented purified sHSPB5-Fc. D, Immunoblot of the purified sHSPB5-Fc in C with antibodies against Fc-tag and HSPB5. Lanes 1–3 represents 20, 40 and 80 ng sHSPB5-Fc proteins respectively. E, Coomassie blue staining sHSPB5-Fc protein that was treated with SDS-loading buffer with (lane 2) or without β-mercaptoethanol. F. Coomassie blue staining sHSPB5-Fc protein that was treated with PNGase F