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Table 4 rPOXA 1B concentrate (enzyme without purification) thermodynamic parameters, calculated during thermal deactivation at study temperatures.

From: Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study

Thermodynamic parameters Temperature K
240.98 ± 5.38 277.40 ± 1.32 297.53 ± 3.88 303.27 ± 1.11 309.58 ± 0.23
Temperature °C
−32.55 ± 4.12 4.32 ± 1.22 24.99 ± 0.25 30.12 ± 1.11 36.43 ± 0.23
Kd (months−1) 3 × 10− 3 0.015 0.055 0.135 0.400
t1/2 (months) 230.8 46.2 12.6 5.1 1.7
Ed (kJ mol−1) 41.40
ΔH (kJ mol−1) 39.40 39.10 38.93 38.88 38.83
ΔG (kJ mol−1) 70.23 77.46 80.04 79.37 78.28
ΔS (J mol−1 K− 1) −127.94 − 138.30 −138.17 − 133.50 − 127.42