Table 4 rPOXA 1B concentrate (enzyme without purification) thermodynamic parameters, calculated during thermal deactivation at study temperatures.
From: Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study
Thermodynamic parameters | Temperature K | ||||
|---|---|---|---|---|---|
240.98 ± 5.38 | 277.40 ± 1.32 | 297.53 ± 3.88 | 303.27 ± 1.11 | 309.58 ± 0.23 | |
Temperature °C | |||||
−32.55 ± 4.12 | 4.32 ± 1.22 | 24.99 ± 0.25 | 30.12 ± 1.11 | 36.43 ± 0.23 | |
Kd (months−1) | 3 × 10− 3 | 0.015 | 0.055 | 0.135 | 0.400 |
t1/2 (months) | 230.8 | 46.2 | 12.6 | 5.1 | 1.7 |
Ed (kJ mol−1) | 41.40 | ||||
ΔH (kJ mol−1) | 39.40 | 39.10 | 38.93 | 38.88 | 38.83 |
ΔG (kJ mol−1) | 70.23 | 77.46 | 80.04 | 79.37 | 78.28 |
ΔS (J mol−1 K− 1) | −127.94 | − 138.30 | −138.17 | − 133.50 | − 127.42 |