Table 3 Successful amplicon, sequencing, and median amplicon length of 11 extraction protocols tested on 6 individual adult, female Teladorsagia circumcincta specimens
Method | Successful Amplicon | Successful Amplicon Sequencing | Median Sequence Length (bp) (Range) |
|---|---|---|---|
AccM | 6/6 (100%) | 1/6 (16.7%) | 93 |
AccW | 5/6 (83.3%) | 1/5 (20%) | 84 |
CheX | 5/6 (83.3%) | 1/5 (20%) | 53 |
CTAB | 1/6 (16.7%) | 1/1 (100%) | 84 |
EznF | 6/6 (100%) | 3/6 (50%) | 72 (58–78) |
IsoG | 6/6 (100%) | 3/6 (50%) | 84 (53–183) |
Schi | 6/6 (100%) | 1/6 (16.7%) | 84 |
Schi-LE | 3/6 (50%) | 2/3 (66.7%) | 135 (84–186) |
SDS | 0/6 (0%) | – | – |
WizM | 3/6 (50%) | 1/3 (33.3%) | 67 |
WizP | 0/6 (0%) | – | – |