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Table 3 Successful amplicon, sequencing, and median amplicon length of 11 extraction protocols tested on 6 individual adult, female Teladorsagia circumcincta specimens

From: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Method Successful Amplicon Successful Amplicon Sequencing Median Sequence Length (bp) (Range)
AccM 6/6 (100%) 1/6 (16.7%) 93
AccW 5/6 (83.3%) 1/5 (20%) 84
CheX 5/6 (83.3%) 1/5 (20%) 53
CTAB 1/6 (16.7%) 1/1 (100%) 84
EznF 6/6 (100%) 3/6 (50%) 72 (58–78)
IsoG 6/6 (100%) 3/6 (50%) 84 (53–183)
Schi 6/6 (100%) 1/6 (16.7%) 84
Schi-LE 3/6 (50%) 2/3 (66.7%) 135 (84–186)
SDS 0/6 (0%)
WizM 3/6 (50%) 1/3 (33.3%) 67
WizP 0/6 (0%)