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Table 2 Median DNA yield (ng/μL), total DNA yield (ng), and quality (A260/280 and A260/230) of 11 extraction protocols tested on 6 individual adult, female Teladorsagia circumcincta specimens

From: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Extraction Method Median (Range) Invitrogen Qubit™ DNA Concentration (ng/μl) Median (Range) Invitrogen Qubit™ DNA Total Yield (ng) Median (Range) NanoDrop 2000™ DNA Concentration (ng/μl) Median (Range) NanoDrop 2000™ DNA Total Yield (ng) Median (Range) NanoDrop 2000™ DNA Quality (A260/280) Median (Range) NanoDrop 2000™ DNA Quality (A260/230)
AccM 0.0897 (0.0228–0.178) 4.485 (2.12–8.9) 0.9 (0.6–1.4) 45 (30–70) 0.935 (−13.1–1.64) 0.53 (0.18–0.73)
AccW 0.08125 (0.0129–0.127) 4.0625 (0.645–6.35) 1.3 (0.9–2.8) 65 (45–140) 1.73 (− 5.76–3.58) 0.77 (0.62–1.52)
CheX 0.98 (0.429–1.64) 49 (24.6–82) 11.5 (9.3–16.5) 575 (465–825) 1.99 (1.63–2.37) 0.535 (0.46–0.67)
CTAB Undetectable Undetectable 0.7 (−1.2–1.1) 35 (− 60–55) 0.675 (− 13.83–1.51) 0.395 (−1.02–0.98)
EznF 0.4325 (0.142–0.672) 21.625 (7.1–40) 4.85 (3.2–11.5) 242.5 (160–575) 2.375 (1.99–2.83) 1.305 (0.84–2.34)
IsoG 0.2035 (0.109–0.372) 10.175 (5.45–18.15) 1.9 (1.0–3.0) 95 (50–150) 1.87 (− 24.56–3.27) 1.545 (0.52–3.51)
Schi 0.962 (0.58–1.51) 48.1 (29–75.7) 6.4 (4.1–8.4) 320 (205–420) 2.19 (1.68–2.77) 0.99 (0.46–2.77)
Schi-LE 0.064 (undetectable – 0.203) 3.08 (undetectable – 10.15) 1.25 (0.5–2.3) 62.5 (25–115) 2.12 (− 6.04–5.39) 0.245 (0.08–0.54)
SDS 0.16 (undetectable – 0.236) 7.425 (undetectable – 11.8) 4.1 (3.3–10.1) 205 (165–505) 3.925 (− 33.69–10.76) 0.05 (0.03–0.11)
WizM 0.0079 (undetectable – 0.0079) 0 (0–0.395) 0.45 (0.2–0.9) 22.5 (10–45) 0.985 (− 3.65–3.78) − 0.005 (− 1.4–0.42)
WizP Undetectable Undetectable 1.4 (0.9–1.8) 70 (45–90) 0.505 (− 12.31–17.77) 0.465 (0.26–1.06)