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Table 2 Kinetic parameters of wild-type and mutated pelN

From: Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability

Enzyme

Kma (g/l)

kcatb (106 s−1)

kcat/Km (105 l/g·s)

Wild type

3.48 ± 0.35

3.29

9.45

K93I

0.74 ± 0.09

0.37

5.05

G241A

3.08 ± 1.25

3.54

11.50

G241V

3.98 ± 2.34

5.45

13.71

K93I/G241A

1.48 ± 0.04

0.78

5.26

  1. a Km, substrate dissociation constant
  2. b kcat, μmol unsaturated product equivalents per second per μmol protein
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BMC Biotechnology

ISSN: 1472-6750

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