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Fig. 3 | BMC Biotechnology

Fig. 3

From: Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

Fig. 3

Visual color changes of developed CL probe-AuNPs hybridization assay at different BVDV-RNA concentrations. Wells 1–5 contain 0.012, 0.124, 1.24, 12.4, and 124 ng/reaction of RNA, respectively (a). Standard curve for the relationship between BVDV-RNA concentrations (ng/reaction) and absorbance ratio (A620/A525) (b) and BVDV concentrations (PFU/reaction) and absorbance ratio (A620/A525) (c). Inserts are the plot of linear correlation for RNA concentrations (1.24 to 12.4 ng/reaction) (b) and for BVDV concentrations (0.14 to 1.05 PFU/reaction) (c). The RNA concentrations 12.4 × 10^ (− 4) ˗ 12.4 × 10^ (− 6) showed absorbance ratio bellow 0.1

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BMC Biotechnology

ISSN: 1472-6750

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