Table 1 Gene primers for Rattus norvegicus with accession number, amplicon size and standard deviation in different cell/tissue types
From: Optimizations for identifying reference genes in bone and cartilage bioengineering
|  | Gene | Accession Number | 5′ sequence | 3′ sequence | Amplicon length (bp) | Precision (Std.Dev.) |
|---|---|---|---|---|---|---|
Reference genes | Tbp | BC081939.1 | TAACCCAGAAAGTCGAAGAC | CCGTAAGGCATCATTGGA | 185 | 0.02a/0.04b/0.13c |
Gapdh | BC083511.1 | CATGGGTGTGAACCATGA | TGTCATGGATGACCTTGG | 104 | 0.04/0.19/0.17 | |
Polr2e | BC158787.1 | GACCATCAAGGTGTACTGC | CAGCTCCTGCTGTAGAAAC | 151 | 0.08/0.16/0.13 | |
Rplp0 | BC001834.2 | CAACCCAGCTCTGGAGA | CAGCTGGCACCTTATTGG | 116 | 0.10/0.05/0.36 | |
Sdha | NM_130428.1 | GCGGTATGACACCAGTTATT | CCTGGCAAGGTAAACCAG | 239 | 0.09/0.07/0.08 | |
Rpl13α | NM_173340.2 | TTTCTCCGAAAGCGGATG | AGGGATCCCATCCAACA | 159 | 0.16/0.03/0.11 | |
Actb | NM_031144.3 | AGCTATGAGCTGCCTGA | GGCAGTAATCTCCTTCTGC | 243 | 0.16/0.33/0.08 | |
Rna28s4 | NR_145822.1 | GCGGCCAAGCGTTCATA | CCTGTCTCACGACGGTCTAA | 143 | 0.10/0.07/0.16 | |
Genes of interest | Tgf-β1 | NM_021578.2 | TTTAGGAAGGACCTGGGTT | ACCCACGTAGTAGACGATG | 210 | 0.04/0.11/0.11 |
Tgf-β3 | NM_013174.2 | AACCTAAGGGTTACTATGCC | ACCACCATGTTGGACAG | 214 | 0.06/0.06/0.08 | |
Sox9 | NM_080403.1 | CCAGAGAACGCACATCAAG | GGTGGTCGGTGTAGTCATA | 161 | 0.07/0.12/0.08 | |
Runx2 | NM_001278484.2 | CCCAAGTGGCCACTTAC | CTGAGGCGGTCAGAGA | 118 | 0.18/0.10/0.32 | |
Acan | NM_022190.1 | CAAGTGGAGCCGTGTTT | GAGCGAAGGTTCTGGATTT | 156 | 0.01/0.13/0.04 | |
Bmp-6 | NM_013107.1 | GGACATGGTCATGAGCTTTG | GTCAGAGTCTCTGTGCTGAT | 232 | 0.43/0.08/0.22 | |
Bmp-2 | NM_017178.1 | GGAAGTGGCCCACTTAGA | TCACTAGCAGTGGTCTTACC | 113 | 0.08/0.09/0.01 | |
Ocn | NM_013414.1 | ACCTGGCAGGTGCAAA | CTCACACACCTCCCTGTG | 237 | 0.03/0.08/0.11 |