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Fig. 1 | BMC Biotechnology

Fig. 1

From: Optimizations for identifying reference genes in bone and cartilage bioengineering

Fig. 1

V0.15- vs. Vmin-based normalization scheme for gene expression assay in rBMSCs undergoing osteogenic differentiation. a Pairwise variation (Vn/n + 1) analysis between NFn and NFn + 1 to determine the optimal number of reference genes required for accurate normalization, V0.15 = 0.078, Vmin = 0.055; b The sequencing of eight candidate reference genes based on M value; c Scatter plots of NFs between V0.15 and Vmin-based scheme (r = Spearman rank correlation coefficient); d The comparison of target gene expression levels (CNRQ) using either the V0.15 or Vmin-based scheme. rBMSCs, rat bone marrow mesenchymal stem cells; NF, normalization factor; CNRQ, calibrated normalized relative quantity

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