Fig. 5

GmCHIT1 promoter expression following hormonal treatments. GFP fluorescence observed in line 131 (pGmCHIT1:GFP), PDF1.2 (pPDF1.2:GFP) or WT detached leaves following hormonal (+) or mock (−) treatments. Treatments were done with coronatine (a) and ACC (b). Graphics represent the mean ± standard errors of fluorescence intensity measured with MetaMorph software via grayscale value on 20 biological replicates. *: significant difference between treated (+) and untreated (−) leaves determined by a Student’s t-test (p < 0.05). Representative images of the observed fluorescence are shown under the graphs. Bar-scales represent 5 mm. Observations were realized at 24, 48 and 72 h after hormonal treatment with a dissection scope (Leica Z16 APO) under GFP filter