Fig. 8

Multi-fragment assembly. a Schematic representation of multi-fragment assembly assessment. We used up to 3 different insert fragments for multi-fragment assembly experiments. Each insert has a 5× molar concentration of the PCR linearized vector. b Effects of the fragment count on transformation. The numbers of transformants and positive clones decreases with an increasing number of fragments. For each transformation, 24 colonies were randomly picked for colony PCR and then subjected to agarose gel electrophoresis analysis. PCR products showing the same band as that of the assembled insert(s) were considered positive