Fig. 5From: Simplified plasmid cloning with a universal MCS design and bacterial in vivo assemblyEffects of insert/vector ratio on assembly efficiency. Effects of insert/vector molar ratio on the recombination of pUC19-UMCS-S-EGFP linearized by PCR (a) or SalI digestion (b) with the mCherry sequence flanked by 15-bp homologous linkers (n = 4)Back to article page