High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate

Table 7 Alkaline serine proteases from G. stearothermophilus and being used to prepare ACE inhibitory hydrolysates

Origin of alkaline serine protease	Expression host	Molecular mass (kDa)	Protease activity (U/mL)^a	Optimal pH and temperature	Hydrolyzed protein^b	IC₅₀ (mg/mL)	Reference
G. stearothermophilus CAU209	B. subtilis WB600	27.2	3807	pH 8.5, 50 °C	Whey protein	0.129	This study
G. stearothermophilus AP-4	–	–	250	pH 9.0, 55 °C	–	–	6
G. stearothermophilus F1	E.coli XL1-Blue	27	1500	pH 9.0, 80 °C	–	–	7
G. stearothermophilus B-1172	E. coli BL21	39	64	pH 9.0, 90 °C	–	–	8
G. stearothermophilus	B. subtilis DB104	35	7020	pH 7.5, 65 °C	–	–	14
Anoxybacillus kamchatkensis M1V	E. coli BL21	28	4600	pH 11.0, 70 °C	Shrimp protein	0.010	44, 45
Aeribacillus pallidus VP3	–	29	3000	pH 10.0, 60 °C	Shrimp protein	0.014	45, 46
Yarrowia lipolytica	–	35	–	pH 9.0, 45 °C	Egg white protein	1.229	47, 48
Sardina pilchardus	–	25	500	pH 8.0, 60 °C	Sardinelle protein	1.200	49, 50
A. clavatus ES1	E. coli BL21	32	260	pH 8.5, 50 °C	Sardinelle protein	7.400	38, 50
B. licheniformis NH1	–	27	1600	pH 10.0, 70 °C	Sardinelle protein	2.100	50, 51

^aProtease activity was measured using casein as a substrate at the optimal conditions for each protease
^bThe protein was hydrolyzed under the optimal hydrolysis condition of each protease
- Not found

ISSN: 1472-6750