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Table 6 Orthogonal experimental results and the optimization of whey protein hydrolysates by GsPros8

From: High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate

Trial

Factor

Peptide content

(%)

pH

Temperature (°C)

Time (h)

Whey protein concentration (%)

1

1

1

1

1

15.6

2

2

1

2

2

17.0

3

3

1

3

3

21.7

4

1

2

2

3

33.4

5

2

2

3

1

29.2

6

3

2

1

2

34.9

7

1

3

3

2

31.4

8

2

3

1

3

51.5

9

3

3

2

1

14.5

K1a

23.5

14.8

21.6

16.4

 

K2a

32.6

32.5

30.7

27.8

 

K3a

23.7

32.5

27.4

35.5

 

Rb

9.1

17.7

9.0

19.1

 

The impact of factors

Whey protein concentration > Temperature > pH > Time

Optimized hydrolysis condition

pH 8.0, 60 °C, 6 h, 11% of whey protein concentration

  1. aK1, K2, and K3 indicated the sum of peptide contents corresponding to level 1, level 2, and level 3, respectively
  2. bR was the correlation coefficient value, R = Max Ki – Min Ki (i = 1, 2, or 3)
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BMC Biotechnology

ISSN: 1472-6750

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