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Table 4 Substrate specificity of GsProS8. Different letters (a-h) on the same column indicated significant (p < 0.05) differences among samples*

From: High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate

Substrate

Specific activity

(U/mg)

Relative activity

(%)

Casein

3560.5 ± 15.8a

100

Whey protein

3277.0 ± 19.8b

92.1

Skim milk powder

3044.8 ± 13.6c

85.5

Hemoglobin

2161.4 ± 54.2d

60.7

Soybean protein isolate

2025.8 ± 45.2d

56.9

Bovine serum albumin

1627.2 ± 22.6e

45.7

Protamine

1333.4 ± 6.8f

37.5

Myoglobin

1312.9 ± 33.9f

36.9

Azocasein

1109.5 ± 0.1g

31.2

Gelatin

374.1 ± 1.6h

10.5

  1. *Enzymatic reaction was carried out using different substrates at 50 °C in 0.05 mol/L MOPS buffer (pH 8.5). Specific activity was shown as mean ± SD (n = 3). Relative activity was expressed as a percentage of the activity when casein as a substrate (the control)
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BMC Biotechnology

ISSN: 1472-6750

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