Fig. 4

a SDS PAGE analysis of novel aldehyde dehydrogenase (DHY-G-VUT7) with the protein band corresponding to a size of 67 kDa after overexpression in BL21 (DE3) E. coli cells following induction at 15 °C for 16 h with 0.5 mM IPTG and purification using the Ni column. Lane 1: Protein Marker, GenScript, Lane 2: BSA (2 μg) and Lane 3: Overexpressed purified DHY-G-VUT7 protein (2 μg). b Western blot analysis of DHY-G-VUT7 protein probed with anti-His antibody. Lane 1: Protein Marker, GenScript, Lane 2: purified His-tagged DHY-G-VUT7 protein of approximately 67 kDa. DHY-G-VUT7 contains a C-terminal 6xHis tag and was purified using Ni-nitrilotriacetic acid (Ni-NTA) column