Fig. 3From: Mining of two novel aldehyde dehydrogenases (DHY-SC-VUT5 and DHY-G-VUT7) from metagenome of hydrocarbon contaminated soilsa SDS PAGE analysis of novel aldehyde dehydrogenase (DHY-SC-VUT5) with the protein band corresponding to a size of 51 kDa after overexpression in BL21 (DE3) E. coli cells following induction at 15 °C for 16 h with 0.5 mM IPTG and purification using the Ni column. Lane 1: Protein Marker, GenScript, Lane 2: BSA (2 μg) and Lane 3: Overexpressed purified DHY-SC-VUT5 protein (2 μg). b Western blot analysis of DHY-SC-VUT5 protein probed with anti-His antibody. Lane 1: Protein Marker, GenScript, Lane 2: purified His-tagged DHY-SC-VUT5 protein of approximately 51 kDa. DHY-SC-VUT5 contains a C-terminal 6xHis tag and was purified using Ni-nitrilotriacetic acid (Ni-NTA) columnBack to article page