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Fig. 3 | BMC Biotechnology

Fig. 3

From: Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)

Fig. 3

Purification and characterization of CsAlaDC and CsSDC. (A) SDS-PAGE analysis of CsAlaDC and CsSDC. Line 1, the molecular-weight marker; line 2, crude enzyme of the E. coli BL21 (DE3) transformed with pET-32a plasmids; line 3, crude enzyme of CsAlaDC; line 4, purified enzyme of CsAlaDC; line 5, crude enzyme of CsSDC; line 6, purified enzyme of CsSDC. (B) Western blot analysis of CsAlaDC and CsSDC. Line 7, crude enzyme of control; line 8, purified CsAlaDC with His-tag; line 9, purified CsSDC with His-tag. (C) Enzyme activity measurement of CsAlaDC and CsSDC in the reaction mixture with or without PLP. (D) Detection of enzyme activities of CsAlaDC and CsSDC by HPLC. (a) Reaction mixture containing Ala and the crude cell lysate of transformants with empty expression vector. (b and c) Reaction mixture containing Ala and the purified CsSDC (b) or CsAlaDC (c). (d–f) corresponding reaction mixture with Ala replaced by Ser

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BMC Biotechnology

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