Fig. 4

Lentiviral constructs function in a transient setting. a The indicated combinations of transient (T) and lentiviral (L) LACE plasmids were transiently transfected into CHO cells and incubated in the dark or with light activation. MFI of eGFP signal was measured by flow cytometry after 24 h of light activation. The 8x gRNA eGFP lentiviral construct was not tested because of its intrinsic eGFP expression in a transient setting (see (b)). b eGFP expression of the 8x gRNA eGFP lentiviral construct. CHO cells were transfected with the lentiviral or transient 8x gRNA eGFP construct. eGFP and bright field images were taken 24 h post-transfection