Fig. 1
From: Transient light-activated gene expression in Chinese hamster ovary cells
Schematic of the LACE system. The LACE system relies on light-activated dimerization of CRY2 and CIBN (blue). CIBN is fused to dCas9 and targeted to the minimal CMV promoter using a synthetic gRNA sequence. CRY2 is fused to VP64 so that it activates transcription following light activation and dimerization with CIBN