Table 1 Plasmids and fungal strain used in this study
From: Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277
| Plasmids/Strain | Genotype | Reference |
|---|---|---|
| Plasmid | ||
| pFC902 | AmpR, pyrG, AMA1, U3-AF crRNA Cas9 | [21] |
| pJET1.2B-KmR | AmpR, kanR | This study |
| pJET-U3P sgRNA | AmpR, U3-AF sgRNA Cas9 | This study |
| pFC333 | AmpR, bleR, AMA1, TEF-Cas9 | [22] |
| pCRISPR0 | AmpR, bleR, AMA1 | This study |
| pCRISPR01 | AmpR, bleR, kanR, AMA1 | This study |
| pCRISPR01-eGFP | AmpR, bleR, kanR, AMA1, TEF1-EGFP | This study |
| pCRISPR01-FnCpf1 | AmpR, bleR, kanR, AMA1, TEF1-FnCpf1 | This study |
| pCRISPR01-FnCpf1-eGFP | AmpR, bleR, kanR, AMA1, TEF1-Cpf1-EGFP fusion | This study |
| pOK-U3P crRNA pyrG-1 | KanR, U3-AF crRNA-pyrG-1 | This study |
| pOK-U3P crRNA pyrG-2 | KanR, U3-AF crRNA-pyrG-2 | This study |
| pOK-U3P crRNA pyrG-3 | KanR, U3-AF crRNA-pyrG-3 | This study |
| pCRISPR01-FnCpf1-pyrG-1 | AmpR, bleR, kanR, AMA1, TEF-Cpf1, crRNA-pyrG-1 | This study |
| pCRISPR01-FnCpf1-pyrG-2 | AmpR, bleR, kanR, AMA1, TEF-Cpf1, crRNA-pyrG-2 | This study |
| pCRISPR01-FnCpf1-pyrG-3 | AmpR, bleR, kanR, AMA1, TEF-Cpf1, crRNA-pyrG-3 | This study |
| Strain | ||
| A. aculeatus TBRC 277 | Wild type | [1] |