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Fig. 2 | BMC Biotechnology

Fig. 2

From: Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

Fig. 2

Schematic of vector constructions for heterologous FnCpf1 expression and confocal microscopy assessment of the subcellular localization of the recombinant eGFP and Cpf1-EGFP fusion proteins in A. aculeatus TBRC 277. a The constructed vectors of (1) pCRISPR01-EGFP, expression of enhanced green fluorescence protein (EGFP), to test the tef1 promoter activity, (2) pCRISPR01-FnCpf1, for subsequent crRNA cassette insertion carrying a protospacer pyrG-targeted locus, (3) pCRISPR01-FnCpf1-EGFP, expression of Cpf1-EGFP, to detect Cpf1 fusion protein. b Subcelullar localization of FnCpf1 as EGFP-fused protein in A. aculeatus TBRC 277. Nuclei were visualized by DAPI staining (blue). Scale bar = 10 μm. DAPI, 4′,6-diamidino-2-phenylindole; EGFP, enhanced green fluorescent protein

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