Table 1 Gene primer sequences for test and reference genes
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
VEGF-A | CTACCAGCGCAGCTATTG | GATCCGCATGATCTGCATAG |
COL4A1 | CTGGGAATCCCGGACTT | GGGATCTCCCTTCATTCCT |
TGF-β1 | TTTAGGAAGGACCTGGGTT | ACCCACGTAGTAGACGATG |
BMP-2 | GGAAGTGGCCCACTTAGA | TCACTAGCAGTGGTCTTACC |
RUNX-2 | CCCAAGTGGCCACTTAC | CTGAGGCGGTCAGAGA |
RPLP0 (reference) | CAACCCAGCTCTGGAGA | CAGCTGGCACCTTATTGG |
SDHA (reference) | GCGGTATGACACCAGTTATT | CCTGGCAAGGTAAACCAG |
POLR2E (reference) | GACCATCAAGGTGTACTGC | CAGCTCCTGCTGTAGAAAC |
TBP (reference) | TAACCCAGAAAGTCGAAGAC | CCGTAAGGCATCATTGGA |