Fig. 6

Maintenance of vascular structure and potential of angiogenesis in tissue pouch models up to 30 days (a-d). Connective tissue grows into the macropore of the scaffold at the periphery (a, dotted lines show the contour of the macropores), with neurovascular bundle still surviving by 30 days (b). Both transcriptional (c) and translational (d) results suggest the maintenance of angiogenesis capacity with a pouch model by 30 days, whereas the capacity is lost with a wrapping model (P < 0.01 and 0.05, respectively). Error bars are Mean ± SEM. *, P < 0.05; **, P < 0.01. H&E staining. M = Skeletal muscle, S = scaffold, CT = connective tissue, MP = macropores, mp = micropores, BV = blood vessel, N = nerve, C = capillary. Bar: A, 200 μm; B, 50 μm