Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

BMC Biotechnology

Table 3 Plasmids

Plasmid^*	Insert
PTRB024	orotidine 5-phosphate decarboxylase (‘Ura3’) with flanking regions
PTRB025	template plasmid to synthesis Ura3 sgRNA
PTRB035	anthranilate synthase component 2 (‘Trp3)’ with flanking regions
PTRB042	template plasmid to synthesis Trp3 sgRNA
PTRB043	template plasmid to synthesis Trp3 sgRNA

^*The complete sequences of the plasmids, including annotation, are given in the Additional file 1

ISSN: 1472-6750