Skip to main content

Table 3 Plasmids

From: Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

Plasmid*Insert
PTRB024orotidine 5-phosphate decarboxylase (‘Ura3’) with flanking regions
PTRB025template plasmid to synthesis Ura3 sgRNA
PTRB035anthranilate synthase component 2 (‘Trp3)’ with flanking regions
PTRB042template plasmid to synthesis Trp3 sgRNA
PTRB043template plasmid to synthesis Trp3 sgRNA
  1. *The complete sequences of the plasmids, including annotation, are given in the Additional file 1