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Table 1 Degree of purity and yield of the rhRSPO1 purification process

From: Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Samples

Efficiency (%)

Purity (%)

Original Conditioned Media

–

3.2

Heparin Affinity Column

100

63

Molecular Exclusion column

52

90

  1. The efficiency of the purification process was calculated by dividing the total mass of rhRSPO1 in each fraction by the initial rhRSPO1 total mass used in the process. rhRSPO1 purity at each step of the purification process was assessed by silver-stained SDS-PAGE and subsequent densitometric analysis using the ImageJ software. The results are presented in percentages. Original Conditioned Medium (Cl.L1); Heparin Affinity Chromatography (Step2); Molecular Exclusion Chromatography (Fractions A10 + A11)
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BMC Biotechnology

ISSN: 1472-6750

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