Fig. 4

Deglycosylation of rhRSPO1 with PNGase F. For each sample 100 ng of purified rhRSPO1 (A10 + A11 fractions from the molecular exclusion chromatography) was applied, under denaturing conditions, previously deglycosylated with N-glycosidase F (PNGase F) or without treatment (NT). a Silver-stained SDS-PAGE analysis of purified rhRSPO1. b Western blot analysis of purified rhRSPO1 using an anti-RSPO1 monoclonal antibody. MWM: Molecular weight marker