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Fig. 1 | BMC Biotechnology

Fig. 1

From: Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Fig. 1

rhRSPO1 purification. a Western Blot analysis of purified rhRSPO1 using an anti-RSPO1 monoclonal antibody. b Silver-stained SDS-PAGE analysis of purified rhRSPO1. Samples: 1- Molecular weight marker; 2- Original conditioned medium from clone Cl.L1; 3- Step2 (after the Heparin Affinity column); 4- A8 fraction of the Molecular Exclusion column; 5- A9 fraction of the Molecular Exclusion column; 6- A10 fraction of the Molecular Exclusion column; 7- A11 fraction of the Molecular Exclusion column; 8- Conditioned medium from HEK293 cells transfected with the empty vector (negative control)

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BMC Biotechnology

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