Fig. 5From: Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assaysThe screening of the constructed yeast library. a. the diploid yeast cells were grown into a typical clover-leaf shape stage; b. the blue positive colonies were grown in stringent QDO plates to increase the selection pressure. +: pGBKT7–53 and pGADT7-T co-transformed as positive control; −: pGBKT7-Lam and pGADT7-T co-transformed as negative control; c. agarose gel electrophoresis of PCR products from selected positive colonies. +: pGADT7-T vector as positive control; −: ddH2O as negative controlBack to article page