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Fig. 3 | BMC Biotechnology

Fig. 3

From: Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays

Fig. 3

The quantification of the library by sequencing of positive colonies and plate counting. a. agarose gel electrophoresis of PCR products from randomly selected 20 colonies. M: DL 2000 DNA Marker; 1–20: PCR products of 20 colonies; +: pGADT7-T vector as positive control; −: ddH2O as negative control. b. plate counting of 100,000-fold diluted bacteria from the E. coli library. c. plate counting of 1000-fold diluted yeast cells from the yeast library

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