Skip to main content

Table 1 Summary of DPIP Purification

From: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin

c

Total

Protiena(mg)

Target proteinb(mg)

Approximate purityc(%)

Yieldd(%)

Cell lysis

200.33

133.82 (CBD-intein-DPIP)

66.8

100

Refolding of fusion protein

156.26

116.73 (CBD-intein-DPIP)

74.7

87.2

Chitin column and intein-mediated cleavage

0.621

0.548 (DPIP)

88.3

2.4

  1. One liter of culture contained about 3.2 g wet weight cells
  2. a: Total protein was quantified by Bradford assay
  3. b: The amount of fusion protein was calculated based on the following formula: target protein = total protein amount x approximate purity
  4. c: The purities of fusion protein were determined by densitometry analysis of their corresponding SDS gel bands
  5. d: The relative low recovery of DPIP was partially due to the low molecular weight of DPIP (6 kDa) compared to the fusion precursor CBD-intein-DPIP (33 kDa)
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us