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Fig. 2 | BMC Biotechnology

Fig. 2

From: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin

Fig. 2

Both temperature and pH affect CI-DPIP cleavage in vitro. a: SDS-PAGE analysis of cleavage products after incubation at different pH and temperature. Lane 1: Input before cleavage reaction, Lanes 2–9: the resin after cleaving by TGE buffer with pH 8.0, 7.5, 7.0, 6.5, 6.0, 5.5, 5.0, 4.5. b: quantified cleavage ratio. All assays were carried out in triplicate and the average was used in this study; the error bars show standard deviations

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