Table 1 Sequences, melting temperature, and amplicon size of primers used for RT-PCR
Target | Sense and antisense sequences 5′ to 3’ | tA (°C) | bp |
|---|---|---|---|
BMP-2 | F: GAGAAGGAGAGGCAAAGAAAG | 61/59 | 183 |
R:GAAGCAGCAACGCTAGAGAC | |||
Osteocalcin | F: ATTGTGGCTCACCCTCCATCA | 60 | 119 |
R: AGGGCTATTTGGGCGTCATC | |||
Osteonectin | F:GCAGAGGAAACCCAAGAGGAG | 60 | 208 |
R: TGGCAAAGAAGTGGCAGCAG | |||
RUNX-2 | F:ACCTTGACCATAACCGTCTTC | 67/57 | 145 |
R: GGCGGTCAGAGAACAAACTA | |||
DSPP | F:CTGGTGCATGAAGGTCATAGAG | 57 | 90 |
R: CAATTTGCGGATCTCAGAGG | |||
GAPDH | F: GAAGGTGAAGGTCGGAGTC | 65 | 100 |
R: GAAGATGGTGATGGGATTTC |