Fig. 3
From: CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells
Transfection efficiency of pSpCas9-2A-GFP plasmid in human primary T cells. Three days after electroporation of activated human primary T cells with no plasmid or pSpCas9-2A-GFP/TRAC gRNA plasmid, the cells were analyzed by flow cytometry in the GFP channel. Analysis of the mock transfected cells was used to estimate the autofluorescence level of the cells in the GFP channel (marked by a bar in the graphs). The fraction of the mock transfected cells above this level (~ 2%) was subtracted from the fraction of the pSpCas9-2A-GFP transfected cells above this level (~ 26%), yielding an estimated transfection efficiency of ~ 24%