Fig. 3

Limonene production using the SC02 strain. a The enantiomeric distribution of limonene was analyzed using the Agilent Technologies 7890B GC System on a Cyclodex B column (30 m × 0.25 mm internal diameter; film thickness = 0.25 μm). GC conditions were as follows: 50 °C hold, 2 °C/min ramping to 160 °C; carrier: high-purity helium, linear velocity: 1 ml/min; temperature of the injector: 250 °C; split ratio: 1:20. Compounds of interest: (S)-(−)-limonene at 21.2 min and (R)-(+)-limonene at 22.02 min. b The fermentation broth was mixed and centrifuged, and the organic layer was analyzed. c The total production of Limonene was measured after 24 h and 48 h of IPTG induction and 10% n-dodecane or DINP induction