Table 1 Details of primers used for T7E1 assay and deep sequencing
From: Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses
Target | Use | Primer sequences (5′-3′) | Product size (bp) |
|---|---|---|---|
Klotho | T7E1 | F: CCTCAAGTAGTAAAACCCTC | 379 |
R: GGTTTTGTCAGCTGACTTAC | |||
Deep sequencing | F: CTTGCTCTTGTCCTCTTTCC | 282 | |
R: CAACAATTCCCCAAGCAAAG |