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Fig. 5 | BMC Biotechnology

Fig. 5

From: Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses

Fig. 5

Sequencing results of 10 potential off-target sites (OTSs) for the sgRNA targeting porcine klotho gene. Direct sequencing of PCR products from wild-type and klotho monoallelic knockout fetal fibroblasts (Fetus V1, V2, and V3) using primers specific to 10 potential OTSs. WT, wild-type; Fetus V1, viable fetus 1 (WT/− 6 bp); Fetus V2, viable fetus 2 (WT/− 17 bp,+ 12 bp); Fetus V3, viable fetus 3 (WT/− 1 bp)

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