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Fig. 4 | BMC Biotechnology

Fig. 4

From: Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses

Fig. 4

Failure to maintain pregnancy in recipients carrying klotho monoallelic knockout fetuses. a. Transfer of cloned embryos reconstructed with klotho monoallelic knockout fetal fibroblasts. b-e. Comparison of expression of aging- and apoptosis-related genes and klotho protein between klotho monoallelic knockout and wild-type placentas. Fetuses along with their placentas were recovered (wild-type, n = 12; Fetus V2, n = 3) between the fourth to fifth week after embryo transfer. Placentas were used for gene expression analysis (wild-type, n = 3; Fetus V2, n = 3). b. IGF1 signaling genes (IGF1 and IGF1R). c. FOXO1 and antioxidant genes (MnSOD and CAT). d. Apoptosis-related genes (BAX/BCL2 ratio and CASPASE3). Within each category, groups marked with different letters are significantly different (P < 0.05). The experiment was replicated three times. e. Expression of klotho protein as detected by Western blot analysis. For improved clarity and conciseness, cropped areas of the blot are shown. All cropped regions originate from the same blot (Additional file 2: Fig. S3). WT, wild-type; Fetus V2, viable fetus 2 (WT/− 17 bp,+ 12 bp); Fetus V3, viable fetus 3 (WT/− 1 bp)

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