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Fig. 3 | BMC Biotechnology

Fig. 3

From: Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses

Fig. 3

Effects of klotho monoallelic knockout on gene expression in fetal fibroblasts and on preimplantation development of embryos cloned from this cell line. a. Comparison of preimplantation development of the cloned embryos reconstructed with wild-type or klotho monoallelic knockout fetal fibroblasts. The experiment was replicated seven times. b-e. Comparison of expression of aging- and apoptosis-related genes and klotho protein between klotho monoallelic knockout and wild-type fetal fibroblasts. Wild-type fetal fibroblasts originated from the syngeneic cells that were used to add the klotho gene modifications. Wild-type and klotho monoallelic knockout fibroblasts were used for gene expression analysis (wild-type, n = 3; Fetus V2, n = 3). b. IGF1 signaling genes (IGF1 and IGF1R). c. FOXO1 and antioxidant genes (MnSOD and CAT). d. Apoptosis-related genes (BAX/BCL2 ratio and CASPASE3). Within each category, groups marked with different letters are significantly different (P < 0.05). The experiment was replicated three times. e. Expression of klotho protein as detected by Western blot analysis. For improved clarity and conciseness, cropped areas of the blot are shown. All cropped regions originate from the same blot (Additional file 2: Fig. S2). WT, wild-type; Fetus V2, viable fetus 2 (WT/− 17 bp,+ 12 bp)

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