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Fig. 2 | BMC Biotechnology

Fig. 2

From: Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses

Fig. 2

Generation of klotho gene knockout fetuses by transfer of embryos cloned from non-selected porcine fibroblasts transfected with Cas9-sgRNA RNPs. a. Results of transfer of cloned embryos reconstructed with non-selected porcine fibroblasts transfected with Cas9-sgRNA RNPs (targeting the klotho gene) to fertile recipients. b. The uterus of the pregnant recipient and c. the 7 viable fetuses at day 28 of gestation. d. Rate of DNA editing on the klotho gene of fetuses recovered after 28 days post embryo transfer. e. A diagram illustrating the editing scheme for exon 3 of the klotho gene of fetuses generated by SCNT using non-selected porcine fibroblasts transfected with Cas9-sgRNA RNPs. Fetus R, resorbing fetus; Fetus V, viable fetus

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