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Table 1 Comparison of decellularization and cytotoxicity of decellularization methods

From: Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro

Decellularization methods   SDS 18 Mean ± SD SDS24 Mean ± SD SDS-T Mean ± SD SDS-T-A Mean ± SD
Residual DNA (%)   10.4 ± 2.35 a 9.13 ± 0.85 b 15.2 ± 1.9 c 7.16 ± 1.6 d
MTT test assay (%) 24 Hours 87.33% ± 28.57 68% ± 20.51 80% ± 9.84 96.33% ± 12.74
7 days 43.33 ± 10.50 e 14% ± 8.32 f 77.66% ± 14.64 g 75% ± 15.71 h
  1. Significant difference between a and c (P < 0.028), b and c (P < 0.054), c and d (P < 0.011), e and f (P < 0.03), e and g (P < 0.014), e and h (P < 0.021), f and g (P < 0.001), and f and h (P < 0.001). The experiments where performed in three replication and results were expressed as mean ± standard deviation