Table 3 Box-Behnken design for xylanase production by Bacillus aestuarii UE25
Run Order | Temperature (°C) | Incubation time (h) | pH | Agitation (rpm) | Inoculum size (%) | Xylanase production (IU mL− 1)a |
|---|---|---|---|---|---|---|
1 | 55 | 24 | 6 | 75 | 7.5 | 0 |
2 | 60 | 24 | 6 | 75 | 7.5 | 14.20 |
3 | 55 | 72 | 6 | 75 | 7.5 | 7.13 |
4 | 60 | 72 | 6 | 75 | 7.5 | 9.15 |
5 | 57.5 | 48 | 5 | 0 | 7.5 | 6.69 |
6 | 57.5 | 48 | 7 | 0 | 7.5 | 17.95 |
7 | 57.5 | 48 | 5 | 150 | 7.5 | 13.32 |
8 | 57.5 | 48 | 7 | 150 | 7.5 | 11.79 |
9 | 57.5 | 24 | 6 | 75 | 5 | 11.70 |
10 | 57.5 | 72 | 6 | 75 | 5 | 9.59 |
11 | 57.5 | 24 | 6 | 75 | 10 | 12.41 |
12 | 57.5 | 72 | 6 | 75 | 10 | 12.76 |
13 | 55 | 48 | 5 | 75 | 7.5 | 0 |
14 | 60 | 48 | 5 | 75 | 7.5 | 6.74 |
15 | 55 | 48 | 7 | 75 | 7.5 | 6.86 |
16 | 60 | 48 | 7 | 75 | 7.5 | 9.33 |
17 | 57.5 | 48 | 6 | 0 | 5 | 10.12 |
18 | 57.5 | 48 | 6 | 150 | 5 | 20.17 |
19 | 57.5 | 48 | 6 | 0 | 10 | 30.10 |
20 | 57.5 | 48 | 6 | 150 | 10 | 12.76 |
21 | 57.5 | 24 | 5 | 75 | 7.5 | 7.74 |
22 | 57.5 | 72 | 5 | 75 | 7.5 | 8.09 |
23 | 57.5 | 24 | 7 | 75 | 7.5 | 8.36 |
24 | 57.5 | 72 | 7 | 75 | 7.5 | 7.39 |
25 | 55 | 48 | 6 | 0 | 7.5 | 11.03 |
26 | 60 | 48 | 6 | 0 | 7.5 | 14.96 |
27 | 55 | 48 | 6 | 150 | 7.5 | 11.53 |
28 | 60 | 48 | 6 | 150 | 7.5 | 22.26 |
29 | 57.5 | 48 | 5 | 75 | 5 | 8.85 |
30 | 57.5 | 48 | 7 | 75 | 5 | 8.80 |
31 | 57.5 | 48 | 5 | 75 | 10 | 9.76 |
32 | 57.5 | 48 | 7 | 75 | 10 | 6.92 |
33 | 55 | 48 | 6 | 75 | 5 | 0 |
34 | 60 | 48 | 6 | 75 | 5 | 16.54 |
35 | 55 | 48 | 6 | 75 | 10 | 15.84 |
36 | 60 | 48 | 6 | 75 | 10 | 12.76 |
37 | 57.5 | 24 | 6 | 0 | 7.5 | 11.35 |
38 | 57.5 | 72 | 6 | 0 | 7.5 | 16.90 |
39 | 57.5 | 24 | 6 | 150 | 7.5 | 8.69 |
40 | 57.5 | 72 | 6 | 150 | 7.5 | 16.66 |
41 | 57.5 | 48 | 6 | 75 | 7.5 | 6.87 |
42 | 57.5 | 48 | 6 | 75 | 7.5 | 8.09 |
43 | 57.5 | 48 | 6 | 75 | 7.5 | 5.87 |
44 | 57.5 | 48 | 6 | 75 | 7.5 | 7.92 |
45 | 57.5 | 48 | 6 | 75 | 7.5 | 8.62 |
46 | 57.5 | 48 | 6 | 75 | 7.5 | 7.50 |