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Table 1 Plackett Burman experimental design (PBD) for xylanase production by using different pretreated sugarcane bagasse

From: Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

Run Order

Bagasse pretreatment

Medium*

Incubation temperature (°C)

pH

Substrate concentration(%)

Inoculumsize (%)

Incubation time (h)

Agitation (150 rpm)

 

PBD 1

PBD 2

PBD 3

       

1

NaOH+ILa

NaOHb

H2O2c

MSM + 0.5% G

60

5

1

5

48

without

2

NaOH+IL

NaOH

H2O2

MSM + 0.5 G & P

55

7

1

5

24

without

3

ILd

H2SO4e

UTBf

MSM + 0.5 G & P

60

5

2

5

24

with

4

NaOH+IL

NaOH

H2O2

MSM + 0.5% G

60

7

1

10

24

with

5

NaOH+IL

NaOH

H2O2

MSM + 0.5 G & P

55

7

2

5

48

with

6

NaOH+IL

NaOH

H2O2

MSM + 0.5 G & P

60

5

2

10

24

without

7

IL

H2SO4

UTB

MSM + 0.5 G & P

60

7

1

10

48

with

8

IL

H2SO4

UTB

MSM + 0.5% G

60

7

2

5

48

without

9

IL

H2SO4

UTB

MSM + 0.5% G

55

7

2

10

24

without

10

NaOH+IL

NaOH

H2O2

MSM + 0.5% G

55

5

2

10

48

with

11

IL

H2SO4

UTB

MSM + 0.5 G & P

55

5

1

10

48

without

12

IL

H2SO4

UTB

MSM + 0.5% G

55

5

1

5

24

with

  1. * MSM Mineral salt medium, G Glucose, P Peptone
  2. a = alkali + ionic liquid, b = alkali, c = hydrogen peroxide, d = ionic liquid, e = acid, f = untreated bagasse.