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Fig. 5 | BMC Biotechnology

Fig. 5

From: Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system

Fig. 5

Co-expression Drosophila chromatin assembly in the presence of DmH1 mRNA

a Production of DmH1 was analyzed by 18% SDS-PAGE and visualized by Coomassie Blue stain. Mr: protein molecular weight marker. b MNase assay was performed for 20 min at 25 °C on assembled Drosophila chromatins prepared in the absence (−) and presence (+) of DmH1 mRNA in the co-expression reaction. MNase digestion was performed right after chromatin assembly for the indicated time, then samples were run on 2.0% agarose gel. Mr: DNA molecular weight marker

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