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Table 3 Fibrinolytic enzyme activity and TDH1 and fib gene copy numbers detected via real-time quantitative PCR

From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Strain Fibase activity Ct value Gene copy (10n) Copy numbers of fib gene (fib/TDH1 copy number)
fib gene TDH1 gene fib gene TDH1 gene
GS115/ fib-5.4c 4428 26.00 ± 0.12 26.22 ± 0.59 18.6 ± 1.46 3.45 ± 1.20 5.4
GS115/ fib-6c 5781 16.13 ± 0.02 16.99 ± 0.23 14.9 ± 1.88 2.50 ± 0.42 6.0
GS115/ fib-8c 7323 16.00 ± 0.19 16.40 ± 0.24 13.4 ± 0.14 1.68 ± 0.27 8.0
GS115/ fib-9c 7930 16.23 ± 0.04 17.19 ± 0.07 12.6 ± 0.32 1.45 ± 0.07 8.7
GS115/ fib-12c 2472 26.35 ± 0.19 27.71 ± 0.19 14.8 ± 1.88 1.21 ± 0.15 12.3
  1. Data are presented as the mean ± SD of triplicate determinations