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Table 3 Fibrinolytic enzyme activity and TDH1 and fib gene copy numbers detected via real-time quantitative PCR

From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Strain

Fibase activity

Ct value

Gene copy (10n)

Copy numbers of fib gene (fib/TDH1 copy number)

fib gene

TDH1 gene

fib gene

TDH1 gene

GS115/ fib-5.4c

4428

26.00 ± 0.12

26.22 ± 0.59

18.6 ± 1.46

3.45 ± 1.20

5.4

GS115/ fib-6c

5781

16.13 ± 0.02

16.99 ± 0.23

14.9 ± 1.88

2.50 ± 0.42

6.0

GS115/ fib-8c

7323

16.00 ± 0.19

16.40 ± 0.24

13.4 ± 0.14

1.68 ± 0.27

8.0

GS115/ fib-9c

7930

16.23 ± 0.04

17.19 ± 0.07

12.6 ± 0.32

1.45 ± 0.07

8.7

GS115/ fib-12c

2472

26.35 ± 0.19

27.71 ± 0.19

14.8 ± 1.88

1.21 ± 0.15

12.3

  1. Data are presented as the mean ± SD of triplicate determinations