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Table 1 The strains and plasmids used in the current study

From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

 

Characteristics

Source

Strains

B. subtilis D21

Wild-type strain with fibrinolytic activity

Lab stock

K. phaffii GS115

His4, host strain, methylotrophic

Lab stock

Plasmids

 Topo-fib

Topo harboring an internal 825-bp fib gene fragment

Current study

 pMD19T-TDH1

pMD19T harboring an internal 1002-bp fib gene fragment

Current study

 pPIC9K

E. coli and K. phaffii shuttle vector; Ampr, G418r containing AOX1 promoter for tightly regulated, methanol-induced expression of the gene

Invitrogen

 pPIC9K-fib

pPIC9K derivative harboring an internal 825-bp fib gene fragment

Current study