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Table 1 The strains and plasmids used in the current study

From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

  Characteristics Source
Strains
B. subtilis D21 Wild-type strain with fibrinolytic activity Lab stock
K. phaffii GS115 His4, host strain, methylotrophic Lab stock
Plasmids
 Topo-fib Topo harboring an internal 825-bp fib gene fragment Current study
 pMD19T-TDH1 pMD19T harboring an internal 1002-bp fib gene fragment Current study
 pPIC9K E. coli and K. phaffii shuttle vector; Ampr, G418r containing AOX1 promoter for tightly regulated, methanol-induced expression of the gene Invitrogen
 pPIC9K-fib pPIC9K derivative harboring an internal 825-bp fib gene fragment Current study