| Characteristics | Source |
---|---|---|
Strains | ||
 B. subtilis D21 | Wild-type strain with fibrinolytic activity | Lab stock |
 K. phaffii GS115 | His4, host strain, methylotrophic | Lab stock |
Plasmids | ||
 Topo-fib | Topo harboring an internal 825-bp fib gene fragment | Current study |
 pMD19T-TDH1 | pMD19T harboring an internal 1002-bp fib gene fragment | Current study |
 pPIC9K | E. coli and K. phaffii shuttle vector; Ampr, G418r containing AOX1 promoter for tightly regulated, methanol-induced expression of the gene | Invitrogen |
 pPIC9K-fib | pPIC9K derivative harboring an internal 825-bp fib gene fragment | Current study |