Fig. 2From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)Effects of codon optimization on fibase activity and yield. After 5 days of cultivation the culture supernatant was harvested and analyzed via a fibase activity assay (b) and SDS-PAGE (c). The fibase purified via one-step Ni-NTA is 46 kD (a). 1: GS115 (pPIC9K). 2: 125 U/ml urokinase. 3: GS115 (pPIC9K-fib). 4: Purified fibase. M: Protein Ruler IIBack to article page