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Table 1 Agrobacterium mediated genetic transformation of embryogenic cell cultures of Citrus sinensis ‘EV2’

From: Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures

Construct Total Somatic Embryos ± SE EGFP+ Somatic Embryos ± SE Transformation Efficiency (%) Lines tested gRNA1 mutation gRNA2 mutation
pC-PDS1 57 15 26.3 12 12 10
pC-PDS2 52 19 36.5 12 12 11