Table 1 Agrobacterium mediated genetic transformation of embryogenic cell cultures of Citrus sinensis ‘EV2’
From: Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures
Construct | Total Somatic Embryos ± SE | EGFP+ Somatic Embryos ± SE | Transformation Efficiency (%) | Lines tested | gRNA1 mutation | gRNA2 mutation |
|---|---|---|---|---|---|---|
pC-PDS1 | 57 | 15 | 26.3 | 12 | 12 | 10 |
pC-PDS2 | 52 | 19 | 36.5 | 12 | 12 | 11 |