Fig. 3
From: Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures
Amplification products obtained from duplex PCR of transgenic ‘EV2’ genomic DNA with gene-specific oligonucleotide primers. A 750 bp fragment of the Cas9 gene was amplified along with a 520 bp fragment of the egfp gene. M, 1 kb marker; 1–6 are six individual transgenic lines containing the pC-PDS1 cassette (upper panel) and the pC-PDS2 cassette (lower panel). PC is positive plasmid DNA control