Table 1 Comparison of published BBMAs for detection of anti-T. gondii IgG antibodies
antigen (final source) | aa | Coupling to beads (X/M) | # reference sera 1 | specificity | sensitivity | antigen/beads | per 1 Mio beads [μg] | signal amplification via biotinylated ab? | Reference |
|---|---|---|---|---|---|---|---|---|---|
SAG1bio-His6 (E. coli) | 31–289 | biotin-streptavidin (M) | 27 / 102 | 1 / 1 | 1 / 1 | 10 μg/ 1.5 × 106 | 6.7 | no | this study |
SAG2Abio-His6 (E. coli) | 27–162 | biotin- streptavidin (M) | 27 / 102 | 1 / 0.98 | 1 / 0.98 | 10 μg/ 1.5 × 106 | 6.7 | no | this study |
SAG1-Stag (E. coli) | 61–300 | chemical (M) | 59 | 0.950 | 0.947 | 30 μg/ 1.25 × 106 | 24 | no | [29] |
GST-SAG2A (E. coli) | 27–173 | chemical (X) | 100 | 1 | 1 | 120 μg/12.5 × 106 | 6 | yes | [10] |
cell lysate (T. gondii) | na | chemical (M) | 20 | 1 | 1 | na | na | no | [46] |
cell lysate (T. gondii) | na | chemical (X) | 80 | 1 | 1 | na | na | yes | [13] |
GST-SAG1 (HeLa cells) | ns | chemical (X) | 5 | 1 | 1 | 5 μg/5 × 106 | 1 | yes | |
GST-SAG1 (E. coli) | 31–349 2 | GSH-casein affinity (X) | 198 | 0.86 | 0.845 | ns | ns | yes | [11] |
GST-SAG2A (E. coli) | 27–187 | GSH-casein affinity (X) | 198 | 0.86 | 0.926 | ns | ns | yes | [11] |